Structural Rearrangements in R432A Variant of AAV2 Affect Genome Packaging
نویسندگان
چکیده
1. Department of Biochemistry & Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL, USA 2. Department of Molecular Genetics and Microbiology, University of Florida Genetics Institute, College of Medicine, University of Florida, Gainesville, FL, USA 3. Department of Chemistry and Biochemistry, and Division of Biological Sciences, University of California—San Diego, San Diego, California, USA
منابع مشابه
Mutational analysis of narrow pores at the fivefold symmetry axes of adeno-associated virus type 2 capsids reveals a dual role in genome packaging and activation of phospholipase A2 activity.
Adeno-associated virus type 2 (AAV2) capsids show 12 pores at the fivefold axes of symmetry. We mutated amino acids which constitute these pores to investigate possible functions of these structures within the AAV2 life cycle. Mutants with alterations in conserved residues were impaired mainly in genome packaging or infectivity, whereas few mutants were affected in capsid assembly. The packagin...
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